- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
esp1-ts
debug: ,
test user =
test db =
Simulation:
Change of parameters: kasesp=0.1, kdiesp=0.002 (=1/500 X WT).
Arrest: Mitotic exit at t=110.7 min.
Experiments:
Cohen-Fix, O. and Koshland, D. (1999). Pds1p of budding yeast has dual roles: inhibition of anaphase initiation and regulation of mitotic exit. Genes Dev. 13:1950-1959.
[Abstract] [Article]
[Abstract] [Article]
Tinker-Kulberg, R.L. and Morgan, D.O. (1999). Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage. Genes Dev. 13:1936-1949.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Tinker-Kulberg, Fig. 3, Mutant is inviable. Cohen-Fix, Fig. 6A, Mutant is inviable, sisters are not separated, but Clb2 is degraded, and the degradation is slightly delayed with a 20 min lag.
Comments: In simulation, the mutant is predicted to be inviable, and Clb2 degradation is delayed by 13 min.
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